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Name
Reconstruct
Byline
Boston University and Medical College of Georgia
Description
Reconstruct is a free editor for serial section microscopy. The software is designed for microscopy in which section resolution is much less than section thickness, such as transmitted electron microscopy (EM) where the resolution is a few nanometers while the section thickness is many tens of nanometers. The program facilitates montaging, cropping, and aligning of images, as well as tracing, measuring, and reconstructing the surface of serially sectioned structures. Three dimensional structures can be previewed in an OpenGL window and saved in VRML format.
NIfTI-1 support
Creator
John C. Fiala
Contact
(fiala@bu.edu)
WWW
synapses.bu.edu
Availability
free
How to get
download from synapses.bu.edu or synapses.mcg.edu
Current version
v1.0.6.0
Current version release date
Jan 26, 2006
Open source
Yes
License
GPL
Available free of charge
Yes
Requirements
Windows (95,98,NT,2000,XP,etc.) Linux with WINE
Technical publications

  • Fiala JC (2005) Reconstruct: a free editor for serial section microscopy. J Microscopy 218:52-61. (PDF reprint)

  • Online Users Manual

  • Fiala JC, Harris KM (2002) Computer-based alignment and reconstruction of serial sections. Microscopy and Analysis. USA Edition. 52:5-7.

  • Fiala JC, Harris KM (2001) Extending unbiased stereology of brain ultrastructure to three-dimensional volumes. Journal of the American Medical Informatics Association 8(1):1-16.

Applications publications

  • Tanaka J, Matsuzaki M, Tarusawa E, Momiyama A, Molnar E, Kasai H, Shigemoto R. (2005) Number and density of AMPA receptors in single synapses in immature cerebellum. J Neurosci. 25(4):799-807.

Other information
Keywords
microscopy, registration, segmentation, stereology, visualization, volume
Platforms
Linux, Windows
IATR listing last updated
30 Jan 2006